Journal: Nutrients

Article Title: Effects of Fructose on Features of Steatotic Liver Disease in HepG2 Cells

doi: 10.3390/nu17172762

Figure Lengend Snippet: Effect of sugars on gene expression of de novo lipogenesis transcription factors and enzymes. HepG2 cells were exposed to vehicle (V), fructose (F, 20 mM), glucose (G, 20 mM) or fatty acids (FA, 110 µM oleic acid, 55 µM palmitic acid) for 6 h. RNA was harvested from cell lysates, and cDNA synthesised. The expression level of NR1H3 ( a ), SREBF1 ( b ), ACACA ( c ) and FASN ( d ) was determined using qPCR. Data from three independent replicates performed in duplicate were normalised to the housekeeper gene ACTB and columns represent a mean ± SD relative to the vehicle control. Statistical significance was analysed using a one-way ANOVA with differences between groups determined using a Bonferroni multiple-comparison post hoc test. * significantly decreased compared to control, p < 0.05.

Article Snippet: The GENEzol TM TriRNA Pure kit, Geneaid DNase I set, Quantabio qScript TM XLT cDNA Supermix, and Quantabio PerfeCTa SYBR Green FastMix were purchased from dnature (Gisborne, New Zealand).

Techniques: Gene Expression, Expressing, Control, Comparison